FRDD
Forensically-Relevant Drug Database
Description
Preferred Drug Name
Brief Description
N-Pyrrolidino ethylene isotonitazene is a synthetic opioid bearing structural resemblance to other nitazene analogues (e.g., N-pyrrolidino isotonitazene, isotonitazene, ethylene etonitazene). NPyrrolidino ethylene isotonitazene differs from currently known nitazene analogues with a lengthened linker between the benzyl and benzimidazole groups.1 N-Pyrrolidino ethylene isotonitazene was first identified by our laboratory in March 2025 and confirmed after acquiring standard reference material.
Nomenclature
Formal Name (IUPAC)
Synonyms
Chemistry
Chemical Formula
Molecular Mass
Molecular Ion
Exact Mass
InChI Key
GCMS Analytics
Lab
Comments
| Sample Preparation: | Drug Materials: Dilution in methanol or acid / base extraction Drug Standards: Dilution in methanol |
| Instrument: | Agilent 5975 Series GC/MSD System |
| Column: | Agilent J&W DB-1 (12 m x 200 µm x 0.33 µm) |
| Carrier Gas: | Helium (Flow: 1.46 mL/min) |
| Temperatures: | Injection Port: 265 °C Transfer Line: 300 °C MS Source: 230 °C MS Quad: 150 °C Oven Program: 50 °C for 0 min, 30 °C/min to 340 °C for 2.3 min |
| Injection Parameters: | Injection Type: Splitless, Injection Volume: 1 µL |
| MS Parameters: | Mass Scan Range: 40-550 m/z |
Images
Fragments
107
42
HRMS Analytics
Lab
Comments
| Sample Preparation: | Toxicology Samples: Liquid-liquid extraction Drug Materials: Dilution of sample in mobile phase |
| Instrument: | Sciex TripleTOF® 5600+, Shimadzu Nexera XR UHPLC Sciex X500R, Sciex ExionLC |
| Column: | Phenomenex® Kinetex C18 (50 mm x 3.0 mm, 2.6 µm) |
| Mobile Phase: | A: Ammonium formate (10 mM, pH 3.0) B: Methanol/acetonitrile (50:50) Flow rate: 0.4 mL/min |
| Gradient: | Initial: 95A:5B; 5A:95B over 13 min; 95A:5B at 15.5 min |
| Temperatures: | Autosampler: 15 °C Column Oven: 30 °C Source Heater: 600 °C |
| Injection Parameters: | Injection Volume: 10 µL |
| QTOF Parameters: | TOF MS Scan Range: 100-510 Da Precursor Isolation: SWATH® acquisition (27 windows) Fragmentation: Collison Energy Spread (35±15 eV) MS/MS Scan Range: 50-510 Da |
Images
Fragments
423.2378
56.0493
