FRDD
Forensically-Relevant Drug Database
Description
Preferred Drug Name
Brief Description
Ethylbromazolam is a designer benzodiazepine and is structurally similar to other known triazolobenzodiazepines (e.g., bromazolam, flubromazolam, phenazolam). Ethylbromazolam has been previously reported in drug materials in Australia and New Zealand.1-3 Ethylbromazolam was first identified by our laboratory in July 2025 and confirmed after acquiring standard reference material.
Structure
Nomenclature
Formal Name (IUPAC)
Identifiers
CAS Numbers
Chemistry
Chemical Formula
Molecular Mass
Molecular Ion
Exact Mass
SMILES
InChI String
InChI Key
GCMS Analytics
Lab
Comments
| Sample Preparation: | Drug Materials: Dilution in methanol or acid / base extraction Drug Standards: Dilution in methanol |
| Instrument: | Agilent 5975 Series GC/MSD System |
| Column: | Agilent J&W DB-1 (12 m x 200 µm x 0.33 µm) |
| Carrier Gas: | Helium (Flow: 1.46 mL/min) |
| Temperatures: | Injection Port: 265 °C Transfer Line: 300 °C MS Source: 230 °C MS Quad: 150 °C Oven Program: 50 °C for 0 min, 30 °C/min to 340 °C for 2.3 min |
| Injection Parameters: | Injection Type: Splitless, Injection Volume: 1 µL |
| MS Parameters: | Mass Scan Range: 40-550 m/z |
Images
Fragments
323
337
HRMS Analytics
Lab
Comments
| Sample Preparation: | Toxicology Samples: Liquid-liquid extraction Drug Materials: Dilution of sample in mobile phase |
| Instrument: | Sciex TripleTOF® 5600+, Shimadzu Nexera XR UHPLC Sciex X500R, Sciex ExionLC |
| Column: | Phenomenex® Kinetex C18 (50 mm x 3.0 mm, 2.6 µm) |
| Mobile Phase: | A: Ammonium formate (10 mM, pH 3.0) B: Methanol/acetonitrile (50:50) Flow rate: 0.4 mL/min |
| Gradient: | Initial: 95A:5B; 5A:95B over 13 min; 95A:5B at 15.5 min |
| Temperatures: | Autosampler: 15 °C Column Oven: 30 °C Source Heater: 600 °C |
| Injection Parameters: | Injection Volume: 10 µL |
| QTOF Parameters: | TOF MS Scan Range: 100-510 Da Precursor Isolation: SWATH® acquisition (27 windows) Fragmentation: Collison Energy Spread (35±15 eV) MS/MS Scan Range: 50-510 Da |
Images
Fragments
339.0356
288.1358
205.0769
